Review



grubii serotype a  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    ATCC grubii serotype a
    A) Experimental design of vaccination regimen and <t>H99</t> challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.
    Grubii Serotype A, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grubii serotype a/product/ATCC
    Average 97 stars, based on 344 article reviews
    grubii serotype a - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Systematic Infectome–Phenome Profiling Reveals Cryptococcal Infection-Associated Proteins Driving Immune System Remodeling and Immunization Potential"

    Article Title: Systematic Infectome–Phenome Profiling Reveals Cryptococcal Infection-Associated Proteins Driving Immune System Remodeling and Immunization Potential

    Journal: bioRxiv

    doi: 10.64898/2026.01.30.702846

    A) Experimental design of vaccination regimen and H99 challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.
    Figure Legend Snippet: A) Experimental design of vaccination regimen and H99 challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.

    Techniques Used: Isolation, Control, Injection, Western Blot, Derivative Assay, Software, Infection



    Similar Products

    grubii serotype a  (ATCC)
    97
    ATCC grubii serotype a
    A) Experimental design of vaccination regimen and <t>H99</t> challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.
    Grubii Serotype A, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grubii serotype a/product/ATCC
    Average 97 stars, based on 1 article reviews
    grubii serotype a - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    serotype a  (ATCC)
    97
    ATCC serotype a
    A) Experimental design of vaccination regimen and <t>H99</t> challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.
    Serotype A, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serotype a/product/ATCC
    Average 97 stars, based on 1 article reviews
    serotype a - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    cryptococcus neoformans serotype a  (ATCC)
    97
    ATCC cryptococcus neoformans serotype a
    C. <t>neoformans</t> and C. deneoformans reference proteomes encode different proteins despite being genetically similar. ( A ) Genome alignment of C. neoformans (CN3) and C. deneoformans (ASM9104v1) genomes was performed using BLAST+ to visualize the distribution of percent nucleotide identity for each alignment. The mean percent nucleotide identity (86.14%) was calculated to determine the overall relatedness between genomes. ( B ) Protein identification from peptides isolated from a serotype A C. neoformans clinical isolate using the C. neoformans (pink, Proteome ID UP000010091) and C. deneoformans (purple, Proteome ID UP000002149) proteomes was performed using DIA-NN to determine the overlap of protein detections. The C. neoformans proteome search detected 3349 proteins and the C. deneoformans search detected 2813 proteins with 1690 proteins (37.7%) shared between both searches.
    Cryptococcus Neoformans Serotype A, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryptococcus neoformans serotype a/product/ATCC
    Average 97 stars, based on 1 article reviews
    cryptococcus neoformans serotype a - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Experimental design of vaccination regimen and H99 challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.

    Journal: bioRxiv

    Article Title: Systematic Infectome–Phenome Profiling Reveals Cryptococcal Infection-Associated Proteins Driving Immune System Remodeling and Immunization Potential

    doi: 10.64898/2026.01.30.702846

    Figure Lengend Snippet: A) Experimental design of vaccination regimen and H99 challenge. Female BALB/c mice ( n = 6 per group) were immunized by intraperitoneal injections of 10 µg EVs isolated from WT or cipC Δ strain in 100 µl of PBS. Control mice were injected with 100 µl PBS, followed by intranasal inoculation of C. neoformans WT. B) Western blot of WT-derived EVs probed by sera from nonimmunized and immunized mice. C) Quantification of serological response to EVs across vaccination regimes. Net protein values obtained from quantification of protein bands in Western blot analysis using ImageJ software. Experiment completed in technical triplicate. Statistical analysis using Students t- test (**, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001). D) Survival rates of vaccinated animals with either WT- or cipC Δ-derived EVs and unvaccinated animals. E) Lung weights of vaccinated and unvaccinated mice infected with C. neoformans WT. F) Spleen weights of vaccinated and unvaccinated mice infected with C. neoformans WT. Statistical analysis using Students t- test (*, P ≤ 0.05). G) Fungal burden from lung, bronchoalveolar lavage, brain, spleen, and liver determined from homogenized tissues and quantification of CFUs. Statistical analysis using Students t- test (**, P ≤ 0.005). H) Cross-reactivity of serological reaction of immunized mice to other major human fungal pathogens, including, including a C. neoformans clinical isolate of strain H99 serotype A, Aspergillus fumigatus ATCC 204305, Candida glabrata BG87, and C. albicans SC3514. Experiment completed in technical duplicate.

    Article Snippet: The derived peak list was incorporated with the Andromeda search engine against the reference C. neoformans var. grubii serotype A (strain H99/ATCC 208821) proteome (7430 sequences; Oct. 2018) and Mus musculus (55,462 sequences; Oct 2018) from Uniprot .

    Techniques: Isolation, Control, Injection, Western Blot, Derivative Assay, Software, Infection

    C. neoformans and C. deneoformans reference proteomes encode different proteins despite being genetically similar. ( A ) Genome alignment of C. neoformans (CN3) and C. deneoformans (ASM9104v1) genomes was performed using BLAST+ to visualize the distribution of percent nucleotide identity for each alignment. The mean percent nucleotide identity (86.14%) was calculated to determine the overall relatedness between genomes. ( B ) Protein identification from peptides isolated from a serotype A C. neoformans clinical isolate using the C. neoformans (pink, Proteome ID UP000010091) and C. deneoformans (purple, Proteome ID UP000002149) proteomes was performed using DIA-NN to determine the overlap of protein detections. The C. neoformans proteome search detected 3349 proteins and the C. deneoformans search detected 2813 proteins with 1690 proteins (37.7%) shared between both searches.

    Journal: Journal of Fungi

    Article Title: Insights from Mass Spectrometry-Based Proteomics on Cryptococcus neoformans

    doi: 10.3390/jof11070529

    Figure Lengend Snippet: C. neoformans and C. deneoformans reference proteomes encode different proteins despite being genetically similar. ( A ) Genome alignment of C. neoformans (CN3) and C. deneoformans (ASM9104v1) genomes was performed using BLAST+ to visualize the distribution of percent nucleotide identity for each alignment. The mean percent nucleotide identity (86.14%) was calculated to determine the overall relatedness between genomes. ( B ) Protein identification from peptides isolated from a serotype A C. neoformans clinical isolate using the C. neoformans (pink, Proteome ID UP000010091) and C. deneoformans (purple, Proteome ID UP000002149) proteomes was performed using DIA-NN to determine the overlap of protein detections. The C. neoformans proteome search detected 3349 proteins and the C. deneoformans search detected 2813 proteins with 1690 proteins (37.7%) shared between both searches.

    Article Snippet: UniProt possesses a Cryptococcus neoformans serotype A (strain H99/ATCC 208821/CBS 10515/FGSC 9487) entry (Taxon ID 235443, Proteome ID UP000010091, 7429 proteins, ), which may better align with commonly used experimental references.

    Techniques: Isolation